Cypermethrin (CYP) is a synthetic pyrethroid II insecticide, used worldwide in different agricultural uses. Chronic human exposure was associated with many health hazards. This study was conducted to evaluate CYP human exposure adverse events including biochemical, hematological and immunological parameters. Leukocytosis and thrombocytosis were common hematological non- significantly changed (P>0.05). Biochemical parameters considered namely SOD, CAT, GSH, GSSG, GPx and TNO levels were determined revealing significant decrease (P<0.05-0.001) compared to normal control samples. Immunophenotyping of T cell subtypes was performed; CD3, CD4 and CD8 count changes were monitored using flow-cytometery where CD3 was significantly elevated in indirectly exposed group (IDEG) (P< 0.05), while CD4 showed non-significant changes in both partially exposed group (PEG)and indirectly exposed group , (P>0.05) , while direct exposed one (DEG) showed a significant decrease (P<0.05). On the other hand CD8 count was significantly elevated in both PEG and IDEG respectively, (P<0.05).Also, CD4/CD8 ratio was significantly reduced in cases of IDEG and PEG (P<0.05- P<0.001). P53 gene sequencing and mutational changes were traced, where exon -5a pattern alternation was detected in 54 %( DEG) and 37 % (IEG) with mutational alignments at nitrogenous base no (56); (T) thymine base replaced with adenine (A) . Both exon-5b and exon-8 showed no changes. In the mean time, tested samples of DEG and PEG showed 18% and 33% pattern changes in both exon-6 and 7 respectively, while exon -7 in case of (IDEG) showed pattern alteration with 37% with mutational alignments at nitrogenous base no (96) ; cytosine(C) replaced with adenine base (A) and also at nitrogenous base no (123) thymine (T) was replaced with adenine base (A).
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